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hscf  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec hscf
    Hscf, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hscf/Mouse+IL-6%2C+premium+grade/bio_rxiv__64898__2026__06__15__732298-57-43-45
    Average 93 stars, based on 28 article reviews
    hscf - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Co-culture Assay:

    Article Title: Triggering the TCR Developmental Checkpoint Activates a Therapeutically Targetable Tumor Suppressive Pathway in T-cell Leukemia
    Article Snippet: For prolonged stimulation, a bead-based assay was used in which cell lines were cultured in 96- or 24-well plates and exposed to 4.5-μm diameter superparamagnetic beads covalently coupled to anti-CD3 and anti-CD28 antibodies (Dynabeads Human T-Activator CD3/CD28, Invitrogen; duration of stimulation from 1 to 12 days). .. Prolonged stimulation of primary T-ALL cells was performed in a coculture assay with confl uent OP9-DL1 in an α-MEM media supplemented with 20% FBS (Hyclone; ThermoFisherScientifi c), 50 μg/mL streptomycin, and 50 IU penicillin and recombinant human cytokines hFLT3L (5 ng/mL), hIL7 (2 ng/ mL), and hSCF (10 ng/mL; Miltenyi). ..

    Recombinant:

    Article Title: Triggering the TCR Developmental Checkpoint Activates a Therapeutically Targetable Tumor Suppressive Pathway in T-cell Leukemia
    Article Snippet: For prolonged stimulation, a bead-based assay was used in which cell lines were cultured in 96- or 24-well plates and exposed to 4.5-μm diameter superparamagnetic beads covalently coupled to anti-CD3 and anti-CD28 antibodies (Dynabeads Human T-Activator CD3/CD28, Invitrogen; duration of stimulation from 1 to 12 days). .. Prolonged stimulation of primary T-ALL cells was performed in a coculture assay with confl uent OP9-DL1 in an α-MEM media supplemented with 20% FBS (Hyclone; ThermoFisherScientifi c), 50 μg/mL streptomycin, and 50 IU penicillin and recombinant human cytokines hFLT3L (5 ng/mL), hIL7 (2 ng/ mL), and hSCF (10 ng/mL; Miltenyi). ..

    Article Title: Targeting IRAK1 in T-Cell acute lymphoblastic leukemia
    Article Snippet: .. Primary T-ALL were co-cultured on OP9-DL1 in a α-MEM media supplemented with 20% FBS (Hyclone ; Thermo Fisher Scientific), 50 μg/ml streptomycin, 50 IU penicillin and recombinant human cytokines hFLT3-L (5 ng/mL), hIL-7 (2 ng/mL) and hSCF (10 ng/mL) (Miltenyi). .. The IRAK1 inhibitor (IRAK1/4 inhibitor or IRAK-Inh; Amgen Inc.) was purchased from Merck Millipore (Ref 407602).

    Article Title: Sensor macrophages derived from human induced pluripotent stem cells to assess pyrogenic contaminations in parenteral drugs.
    Article Snippet: Ensuring the safety of parenteral drugs before injection into patients is of utmost importance.. New regulations around the globe and the need to refrain from using animals however, have highlighted the need for new cell sources to be used in next-generation bioassays to detect the entire spectrum of possible contaminating pyrogens.. Given the current drawbacks of the Monocyte-Activation-Test (MAT) with respect to the use of primary peripheral blood mono-nuclear cells or the use of monocytic cell lines, we here demonstrate the manufacturing of sensor monocytes/macrophages from human induced pluripotent stem cells (iMonoMac), which are fully defined and superior to current cell products.

    Concentration Assay:

    Article Title: CRISPR-Mediated Linearization of IDLV Donor Enables Precise Targeted Integration in Human Hematopoietic Stem Cells
    Article Snippet: G-CSF mobilized PB CD34 + HSPCs were purchased frozen by CliniSciences. .. HPSCs were seeded at the concentration of 5×10 5 cells per ml in prestimulation media: serum-free StemSpan SFEM (Cat#09650, STEMCELL Technologies) supplemented with 100 U/ml penicillin, 100 g/ml streptomycin (Cat#15140-122, GIBCO TM ), 2 mM GlutaMax (Cat# 35050038, GIBCO TM ), 300 ng/ml hSCF (Cat#130-096-685, Milteny Biotech), 300 ng/ml hFlt3-L (Cat#130-096-479, Milteny Biotech), 100 ng/ml hTPO (Cat#130-095-752, Milteny Biotech), 20 ng/ml IL3 (Cat#130-095-069, Milteny Biotech), 750 nM and Stemregenin-1 (Cat#72342, STEMCELL Technologies) and 350 μM UM171 (Cat#72912, STEMCELL Technologies), 10 μM 16,16-Dimethyl Prostaglandin E2 (dmPGE2; Cat#14750, Cayman Chemical Company) and 4 μg/ml Protamine Sulfate (Cat# P3369, Sigma Aldrich). dmPGE2 and protamine sulfate were added at the beginning of the culture, 2 h prior to IDLV transduction. ..

    Transduction:

    Article Title: CRISPR-Mediated Linearization of IDLV Donor Enables Precise Targeted Integration in Human Hematopoietic Stem Cells
    Article Snippet: G-CSF mobilized PB CD34 + HSPCs were purchased frozen by CliniSciences. .. HPSCs were seeded at the concentration of 5×10 5 cells per ml in prestimulation media: serum-free StemSpan SFEM (Cat#09650, STEMCELL Technologies) supplemented with 100 U/ml penicillin, 100 g/ml streptomycin (Cat#15140-122, GIBCO TM ), 2 mM GlutaMax (Cat# 35050038, GIBCO TM ), 300 ng/ml hSCF (Cat#130-096-685, Milteny Biotech), 300 ng/ml hFlt3-L (Cat#130-096-479, Milteny Biotech), 100 ng/ml hTPO (Cat#130-095-752, Milteny Biotech), 20 ng/ml IL3 (Cat#130-095-069, Milteny Biotech), 750 nM and Stemregenin-1 (Cat#72342, STEMCELL Technologies) and 350 μM UM171 (Cat#72912, STEMCELL Technologies), 10 μM 16,16-Dimethyl Prostaglandin E2 (dmPGE2; Cat#14750, Cayman Chemical Company) and 4 μg/ml Protamine Sulfate (Cat# P3369, Sigma Aldrich). dmPGE2 and protamine sulfate were added at the beginning of the culture, 2 h prior to IDLV transduction. ..

    Cell Culture:

    Article Title: Sensor macrophages derived from human induced pluripotent stem cells to assess pyrogenic contaminations in parenteral drugs.
    Article Snippet: Ensuring the safety of parenteral drugs before injection into patients is of utmost importance.. New regulations around the globe and the need to refrain from using animals however, have highlighted the need for new cell sources to be used in next-generation bioassays to detect the entire spectrum of possible contaminating pyrogens.. Given the current drawbacks of the Monocyte-Activation-Test (MAT) with respect to the use of primary peripheral blood mono-nuclear cells or the use of monocytic cell lines, we here demonstrate the manufacturing of sensor monocytes/macrophages from human induced pluripotent stem cells (iMonoMac), which are fully defined and superior to current cell products.



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